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bplf1 aa 1 325  (Addgene inc)


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    Addgene inc bplf1 aa 1 325
    Bplf1 Aa 1 325, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/prl+tk+let7+b/pRL-TK+let7+B+(Plasmid+%2311325)/pm34543352-182-11-48
    Average 90 stars, based on 4 article reviews
    bplf1 aa 1 325 - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: Dynamic recruitment of single RNAs to processing bodies depends on RNA functionality
    Article Snippet: .. Plasmids pRL-TK-let7-A, pRL-TK-let7-B, pRL-TK-cxcr4–6x, phage-ubc-nls-ha-2xmcp-HALO (a gift from Phil Sharp, Addgene plasmid # 11324, #11325, # 11308 and # 64540) and pmiR-GLO (pmG, Promega, # E1330) were purchased. pmG-MS2, encoding the firefly luciferase (FL) gene followed by 24 MS2 stem loops (FL-MS2), was created in two steps. ..

    Article Title: Dynamic Recruitment of Single RNAs to Processing Bodies Depends on RNA Functionality.
    Article Snippet: .. Plasmids pRL-TK-let7-A, pRL-TK-let7-B, pRL-TK-cxcr4-6x, phage-ubc-nls-ha2xmcp-HALO (a gift from Phil Sharp, Addgene plasmid # 11324, #11325, # 11308 and # 64540) and pmiR-GLO (pmG, Promega, # E1330) were purchased. pmG-MS2, encoding the firefly luciferase (FL) gene followed by 24MS2 stem loops (FL-MS2), was created in two steps. ..

    Article Title: Dynamic recruitment of single RNAs to processing bodies depends on RNA functionality
    Article Snippet: .. Plasmids pRL-TK-let7-A, pRL-TK-let7-B, pRL-TK-cxcr4-6x, phage-ubc-nls-ha-2xmcp-HALO (a gift from Phil Sharp, Addgene plasmid # 11324, #11325, # 11308 and # 64540) and pmiR-GLO (pmG, Promega, # E1330) were purchased. pmG-MS2, encoding the firefly luciferase (FL) gene followed by 24 MS2 stem loops (FL-MS2), was created in two steps. ..

    Luciferase:

    Article Title: Dynamic recruitment of single RNAs to processing bodies depends on RNA functionality
    Article Snippet: .. Plasmids pRL-TK-let7-A, pRL-TK-let7-B, pRL-TK-cxcr4–6x, phage-ubc-nls-ha-2xmcp-HALO (a gift from Phil Sharp, Addgene plasmid # 11324, #11325, # 11308 and # 64540) and pmiR-GLO (pmG, Promega, # E1330) were purchased. pmG-MS2, encoding the firefly luciferase (FL) gene followed by 24 MS2 stem loops (FL-MS2), was created in two steps. ..

    Article Title: Dynamic Recruitment of Single RNAs to Processing Bodies Depends on RNA Functionality.
    Article Snippet: .. Plasmids pRL-TK-let7-A, pRL-TK-let7-B, pRL-TK-cxcr4-6x, phage-ubc-nls-ha2xmcp-HALO (a gift from Phil Sharp, Addgene plasmid # 11324, #11325, # 11308 and # 64540) and pmiR-GLO (pmG, Promega, # E1330) were purchased. pmG-MS2, encoding the firefly luciferase (FL) gene followed by 24MS2 stem loops (FL-MS2), was created in two steps. ..

    Article Title: Dynamic recruitment of single RNAs to processing bodies depends on RNA functionality
    Article Snippet: .. Plasmids pRL-TK-let7-A, pRL-TK-let7-B, pRL-TK-cxcr4-6x, phage-ubc-nls-ha-2xmcp-HALO (a gift from Phil Sharp, Addgene plasmid # 11324, #11325, # 11308 and # 64540) and pmiR-GLO (pmG, Promega, # E1330) were purchased. pmG-MS2, encoding the firefly luciferase (FL) gene followed by 24 MS2 stem loops (FL-MS2), was created in two steps. ..



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    camkiiα 1 325  (Addgene inc)
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    Addgene inc camkiiα 1 325
    A, Schematic of paAIP2. LOV2-Jα domain derived from phototropin1 was fused to autocamtide-2 related inhibitory peptide 2 (AIP2). The blue light absorption causes a reversible conformational change of LOV2 domain, leading to dissociation of the Jα helix-AIP2. The structurally released AIP2 binds to the kinase domain of <t>CaMKII</t> and inhibits its kinase activity.
    Camkiiα 1 325, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    A, Schematic of paAIP2. LOV2-Jα domain derived from phototropin1 was fused to autocamtide-2 related inhibitory peptide 2 (AIP2). The blue light absorption causes a reversible conformational change of LOV2 domain, leading to dissociation of the Jα helix-AIP2. The structurally released AIP2 binds to the kinase domain of CaMKII and inhibits its kinase activity.

    Journal: Neuron

    Article Title: Kinetics of endogenous CaMKII required for synaptic plasticity revealed by optogenetic kinase inhibitor

    doi: 10.1016/j.neuron.2017.02.036

    Figure Lengend Snippet: A, Schematic of paAIP2. LOV2-Jα domain derived from phototropin1 was fused to autocamtide-2 related inhibitory peptide 2 (AIP2). The blue light absorption causes a reversible conformational change of LOV2 domain, leading to dissociation of the Jα helix-AIP2. The structurally released AIP2 binds to the kinase domain of CaMKII and inhibits its kinase activity.

    Article Snippet: Protein purification from HEK293 cells For construction of plasmids, CaMKIIα 1–325 , CaMKI 1–319 (purchased from Kazusa), CaMKIV 1–337 (a gift from Richard Maurer, Addgene plasmid # 45062) ( Sun et al., 1994 ), PKA 43–331 (synthesized), PKC 338–618 (a gift from Alex Toker, Addgene plasmid #10805) ( Hodges et al., 2002 ), PAK3 247–544 (a gift from William Hahn & David Root, Addgene plasmid # 23439) ( Johannessen et al., 2010 ), and ROCK 287–390 (purchased from Kazusa) genes were inserted into the modified pEGFP-C1 vector with His-tagged mEGFP, respectively.

    Techniques: Derivative Assay, Activity Assay

    A, Effect of paAIP2 under blue-light was tested on different kinases. Phosphorylation of purified Syn-1 peptide by various active kinases was detected by western blotting. PaAIP2 blocks CaMKII activity at various concentrations, while having no effect on other kinases.

    Journal: Neuron

    Article Title: Kinetics of endogenous CaMKII required for synaptic plasticity revealed by optogenetic kinase inhibitor

    doi: 10.1016/j.neuron.2017.02.036

    Figure Lengend Snippet: A, Effect of paAIP2 under blue-light was tested on different kinases. Phosphorylation of purified Syn-1 peptide by various active kinases was detected by western blotting. PaAIP2 blocks CaMKII activity at various concentrations, while having no effect on other kinases.

    Article Snippet: Protein purification from HEK293 cells For construction of plasmids, CaMKIIα 1–325 , CaMKI 1–319 (purchased from Kazusa), CaMKIV 1–337 (a gift from Richard Maurer, Addgene plasmid # 45062) ( Sun et al., 1994 ), PKA 43–331 (synthesized), PKC 338–618 (a gift from Alex Toker, Addgene plasmid #10805) ( Hodges et al., 2002 ), PAK3 247–544 (a gift from William Hahn & David Root, Addgene plasmid # 23439) ( Johannessen et al., 2010 ), and ROCK 287–390 (purchased from Kazusa) genes were inserted into the modified pEGFP-C1 vector with His-tagged mEGFP, respectively.

    Techniques: Phospho-proteomics, Purification, Western Blot, Activity Assay

    A, Schematic of the experiment to measure binding between CaMKII and paAIP2.

    Journal: Neuron

    Article Title: Kinetics of endogenous CaMKII required for synaptic plasticity revealed by optogenetic kinase inhibitor

    doi: 10.1016/j.neuron.2017.02.036

    Figure Lengend Snippet: A, Schematic of the experiment to measure binding between CaMKII and paAIP2.

    Article Snippet: Protein purification from HEK293 cells For construction of plasmids, CaMKIIα 1–325 , CaMKI 1–319 (purchased from Kazusa), CaMKIV 1–337 (a gift from Richard Maurer, Addgene plasmid # 45062) ( Sun et al., 1994 ), PKA 43–331 (synthesized), PKC 338–618 (a gift from Alex Toker, Addgene plasmid #10805) ( Hodges et al., 2002 ), PAK3 247–544 (a gift from William Hahn & David Root, Addgene plasmid # 23439) ( Johannessen et al., 2010 ), and ROCK 287–390 (purchased from Kazusa) genes were inserted into the modified pEGFP-C1 vector with His-tagged mEGFP, respectively.

    Techniques: Binding Assay

    A, Averaged time course of spine volume change under different temporal inhibition of CaMKII. During single-spine stimulation with 2-photon glutamate uncaging (0.5 Hz, 30 pulses), neurons were illuminated with blue light for 1 min with different timing relative to the onset of glutamate uncaging (Time shift 0, 10, 30, 60 s). Fluorescence intensity of mEGFP was used to measure the spine volume change. The numbers of samples are indicated in the panels.

    Journal: Neuron

    Article Title: Kinetics of endogenous CaMKII required for synaptic plasticity revealed by optogenetic kinase inhibitor

    doi: 10.1016/j.neuron.2017.02.036

    Figure Lengend Snippet: A, Averaged time course of spine volume change under different temporal inhibition of CaMKII. During single-spine stimulation with 2-photon glutamate uncaging (0.5 Hz, 30 pulses), neurons were illuminated with blue light for 1 min with different timing relative to the onset of glutamate uncaging (Time shift 0, 10, 30, 60 s). Fluorescence intensity of mEGFP was used to measure the spine volume change. The numbers of samples are indicated in the panels.

    Article Snippet: Protein purification from HEK293 cells For construction of plasmids, CaMKIIα 1–325 , CaMKI 1–319 (purchased from Kazusa), CaMKIV 1–337 (a gift from Richard Maurer, Addgene plasmid # 45062) ( Sun et al., 1994 ), PKA 43–331 (synthesized), PKC 338–618 (a gift from Alex Toker, Addgene plasmid #10805) ( Hodges et al., 2002 ), PAK3 247–544 (a gift from William Hahn & David Root, Addgene plasmid # 23439) ( Johannessen et al., 2010 ), and ROCK 287–390 (purchased from Kazusa) genes were inserted into the modified pEGFP-C1 vector with His-tagged mEGFP, respectively.

    Techniques: Inhibition, Fluorescence